Enzymatic reactions on immobilised substrates

This review gives an overview of enzymatic reactions that have been conducted on substrates attached to solid surfaces. Such biochemical reactions have become more important with the drive to miniaturisation and automation in chemistry, biology and medicine. Technical aspects such as choice of solid surface and analytical methods are discussed and examples of enzyme reactions that have been successful on these surfaces are provided.

Christopher J. Gray,  Martin J. Weissenborn, Claire E. Eyers and  Sabine L. Flitsch

Chem. Soc. Rev., 2013,42, 6378-6405
DOI: 10.1039/C3CS60018A
Received 19 Jan 2013, First published online 11 Apr 2013