{"id":521,"date":"2018-10-15T12:51:24","date_gmt":"2018-10-15T12:51:24","guid":{"rendered":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/?p=521"},"modified":"2019-07-08T09:44:03","modified_gmt":"2019-07-08T09:44:03","slug":"origami-a-software-suite-for-activated-ion-mobility-mass-spectrometry-aim-ms-applied-to-multimeric-protein-assemblies","status":"publish","type":"post","link":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/2018\/10\/15\/origami-a-software-suite-for-activated-ion-mobility-mass-spectrometry-aim-ms-applied-to-multimeric-protein-assemblies\/","title":{"rendered":"ORIGAMI: A software suite for activated ion mobility mass spectrometry (aIM-MS) applied to multimeric protein assemblies"},"content":{"rendered":"

We present here a software suite (ORIGAMI<\/strong>) that facilitates the rapid acquisition and analysis of ion mobility data following\u00a0collisional activation<\/a>.\u00a0ORIGAMI<\/strong>\u00a0was developed for use on Waters Synapt instruments where data acquisition is achieved by interfacing WREnS (Waters Research Enabled Software) and MassLynx. Two components are presented, the first is\u00a0ORIGAMI<\/strong>MS<\/sup><\/em>\u00a0which enables the activation of ions via a sequential ramping of collision voltage prior to ion mobility analysis. We demonstrate the use of\u00a0ORIGAMI<\/strong>\u00a0on the tetrameric assemblies formed by the proteins concanavalin A (103\u00a0kDa) and\u00a0alcohol dehydrogenase<\/a>(143\u00a0kDa). Activation is performed in the trap collision cell of the Synapt TriWave assembly where the collision voltage can be ramped from 0 to 200\u00a0V. All of the acquired data is recorded in a single file which simplifies data acquisition while keeping the acquisition times at approx. 25\u00a0min for a single charge state exposed to a collision voltage range of 0\u2013200\u00a0V. Following acquisition, the data are analysed in the second component,\u00a0ORIGAMI<\/strong>ANALYSE<\/sup><\/em>, which allows the user to visualise the effect of activation on the mobility of the parent ion, as well as on any fragment ion. The user can export the data in the form of heat maps, waterfall or wire plots as well as interactive and shareable webpages. In addition, tools implemented in\u00a0ORIGAMI<\/strong>\u00a0enable easy data extraction from single or multiple MassLynx .raw files, in-depth interrogation of large datasets, statistical analysis and figure creation capabilities.<\/p>\n

https:\/\/doi.org\/10.1016\/j.ijms.2017.08.014<\/a><\/p>\n

\"\"<\/p>\n

Lukasz G.<\/span>Migas,\u00a0<\/span><\/span>Aidan P.<\/span>France,\u00a0<\/span><\/span>Bruno<\/span>Bellina and\u00a0<\/span><\/span>Perdita E.<\/span>Barran<\/span><\/span><\/em><\/p>\n","protected":false},"excerpt":{"rendered":"

We present here a software suite (ORIGAMI) that facilitates the rapid acquisition and analysis of ion mobility data following\u00a0collisional activation.\u00a0ORIGAMI\u00a0was developed for use on Waters Synapt instruments where data acquisition is achieved by interfacing WREnS (Waters Research Enabled Software) and MassLynx. Two components are presented, the first is\u00a0ORIGAMIMS\u00a0which enables the activation of ions via a … <\/p>\n

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