{"id":383,"date":"2015-10-09T16:10:25","date_gmt":"2015-10-09T16:10:25","guid":{"rendered":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/?p=383"},"modified":"2018-10-15T12:57:37","modified_gmt":"2018-10-15T12:57:37","slug":"distinguishing-loss-of-structure-from-subunit-dissociation-for-protein-complexes-with-variable-temperature-ion-mobility-mass-spectrometry","status":"publish","type":"post","link":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/2015\/10\/09\/distinguishing-loss-of-structure-from-subunit-dissociation-for-protein-complexes-with-variable-temperature-ion-mobility-mass-spectrometry\/","title":{"rendered":"Distinguishing Loss of Structure from Subunit Dissociation for Protein Complexes with Variable Temperature Ion Mobility Mass Spectrometry"},"content":{"rendered":"<p>Proteins and protein complexes undergo structural changes depending on the surrounding environment, in turn affecting their biological function.\u00a0 Here, VT-MS is applied to study four multimeric protein complexes and allow for decoupling of their melting temperature (T<sub>m<\/sub>) from the protein complex dissociation temperature (T<sub>GPD<\/sub>).\u00a0\u00a0 VT-IM-MS is used to investigate structural changes of these proteins at elevated temperatures and provide insight into the thermally induced dissociation (TID) mechanism, as well as strength of the non-covalent interactions between subunits.<\/p>\n<p><a href=\"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1_2.png?ssl=1\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\"wp-image-380 size-full aligncenter\" src=\"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1_2.png?resize=486%2C318&#038;ssl=1\" alt=\"kam1_pap1_2\" width=\"486\" height=\"318\" srcset=\"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1_2.png?w=486&amp;ssl=1 486w, https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1_2.png?resize=293%2C192&amp;ssl=1 293w, https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1_2.png?resize=300%2C196&amp;ssl=1 300w\" sizes=\"auto, (max-width: 486px) 100vw, 486px\" \/><\/a><\/p>\n<p>&nbsp;<\/p>\n<blockquote><p><a href=\"http:\/\/pubs.acs.org\/doi\/abs\/10.1021\/acs.analchem.5b01063\"><strong>Distinguishing Loss of Structure from Subunit Dissociation for Protein Complexes with Variable Temperature Ion Mobility Mass Spectrometry<\/strong><\/a><\/p>\n<p>Kamila J. Pacholarz and Perdita E. Barran<\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>Proteins and protein complexes undergo structural changes depending on the surrounding environment, in turn affecting their biological function.\u00a0 Here, VT-MS is applied to study four multimeric protein complexes and allow for decoupling of their melting temperature (Tm) from the protein complex dissociation temperature (TGPD).\u00a0\u00a0 VT-IM-MS is used to investigate structural changes of these proteins at &hellip; <\/p>\n<p><a class=\"more-link btn\" href=\"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/2015\/10\/09\/distinguishing-loss-of-structure-from-subunit-dissociation-for-protein-complexes-with-variable-temperature-ion-mobility-mass-spectrometry\/\">Continue reading<\/a><\/p>\n","protected":false},"author":2,"featured_media":380,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[3],"tags":[],"class_list":["post-383","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications","item-wrap"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1_2.png?fit=486%2C318&ssl=1","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4Adwh-6b","_links":{"self":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts\/383","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/comments?post=383"}],"version-history":[{"count":2,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts\/383\/revisions"}],"predecessor-version":[{"id":395,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts\/383\/revisions\/395"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/media\/380"}],"wp:attachment":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/media?parent=383"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/categories?post=383"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/tags?post=383"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}