{"id":379,"date":"2015-10-09T16:08:47","date_gmt":"2015-10-09T16:08:47","guid":{"rendered":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/?p=379"},"modified":"2018-10-15T12:57:28","modified_gmt":"2018-10-15T12:57:28","slug":"signature-dynamics-of-intact-immunoglobulin-g-revealed-by-drift-tube-ion-mobility-mass-spectrometry-and-molecular-modeling","status":"publish","type":"post","link":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/2015\/10\/09\/signature-dynamics-of-intact-immunoglobulin-g-revealed-by-drift-tube-ion-mobility-mass-spectrometry-and-molecular-modeling\/","title":{"rendered":"Signature dynamics of intact immunoglobulin G revealed by drift-tube ion-mobility mass spectrometry and molecular modeling"},"content":{"rendered":"<p>Monoclonal antibodies (mAb) are a rapidly growing group of biopharmaceuticals.\u00a0 Due to their intrinsic flexibility, intact mAbs provide a challenge with regards to higher order structure characterization.\u00a0 Intact mAbs and their fragments are explored here using the DT-IM-MS and were found to be far more dynamic in the gas-phase than proteins of comparable size.\u00a0 This is rationalised with MD simulations, which revealed dynamics between linked folded domains of mAbs.<\/p>\n<p><a href=\"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1.png?ssl=1\"><img data-recalc-dims=\"1\" loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-381 size-full\" src=\"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1.png?resize=602%2C431&#038;ssl=1\" alt=\"kam1_pap1\" width=\"602\" height=\"431\" srcset=\"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1.png?w=602&amp;ssl=1 602w, https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1.png?resize=268%2C192&amp;ssl=1 268w, https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1.png?resize=300%2C215&amp;ssl=1 300w\" sizes=\"auto, (max-width: 602px) 100vw, 602px\" \/><\/a><\/p>\n<blockquote><p><strong>Signature dynamics of intact immunoglobulin G revealed by drift-tube ion-mobility mass spectrometry and molecular modeling<\/strong><\/p>\n<p>Kamila J. Pacholarz, Massimiliano Porrini, Rachel A. Garlish, Richard J. Taylor, Alistair J. Henry, and Perdita E. Barran<\/p><\/blockquote>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Monoclonal antibodies (mAb) are a rapidly growing group of biopharmaceuticals.\u00a0 Due to their intrinsic flexibility, intact mAbs provide a challenge with regards to higher order structure characterization.\u00a0 Intact mAbs and their fragments are explored here using the DT-IM-MS and were found to be far more dynamic in the gas-phase than proteins of comparable size.\u00a0 This &hellip; <\/p>\n<p><a class=\"more-link btn\" href=\"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/2015\/10\/09\/signature-dynamics-of-intact-immunoglobulin-g-revealed-by-drift-tube-ion-mobility-mass-spectrometry-and-molecular-modeling\/\">Continue reading<\/a><\/p>\n","protected":false},"author":2,"featured_media":381,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2}},"categories":[3],"tags":[],"class_list":["post-379","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publications","item-wrap"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/www.mbc.manchester.ac.uk\/barrangroup\/wp-content\/uploads\/sites\/2\/2015\/10\/kam1_pap1.png?fit=602%2C431&ssl=1","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p4Adwh-67","_links":{"self":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts\/379","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/comments?post=379"}],"version-history":[{"count":1,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts\/379\/revisions"}],"predecessor-version":[{"id":382,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/posts\/379\/revisions\/382"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/media\/381"}],"wp:attachment":[{"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/media?parent=379"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/categories?post=379"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.mbc.manchester.ac.uk\/barrangroup\/wp-json\/wp\/v2\/tags?post=379"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}