Oct 09

Signature dynamics of intact immunoglobulin G revealed by drift-tube ion-mobility mass spectrometry and molecular modeling

Monoclonal antibodies (mAb) are a rapidly growing group of biopharmaceuticals.  Due to their intrinsic flexibility, intact mAbs provide a challenge with regards to higher order structure characterization.  Intact mAbs and their fragments are explored here using the DT-IM-MS and were found to be far more dynamic in the gas-phase than proteins of comparable size.  This is rationalised with MD simulations, which revealed dynamics between linked folded domains of mAbs.

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Signature dynamics of intact immunoglobulin G revealed by drift-tube ion-mobility mass spectrometry and molecular modeling

Kamila J. Pacholarz, Massimiliano Porrini, Rachel A. Garlish, Richard J. Taylor, Alistair J. Henry, and Perdita E. Barran

 

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