Distinguishing Loss of Structure from Subunit Dissociation for Protein Complexes with Variable Temperature Ion Mobility Mass Spectrometry

Proteins and protein complexes undergo structural changes depending on the surrounding environment, in turn affecting their biological function.  Here, VT-MS is applied to study four multimeric protein complexes and allow for decoupling of their melting temperature (Tm) from the protein complex dissociation temperature (TGPD).   VT-IM-MS is used to investigate structural changes of these proteins at elevated temperatures and provide insight into the thermally induced dissociation (TID) mechanism, as well as strength of the non-covalent interactions between subunits.

kam1_pap1_2

 

Distinguishing Loss of Structure from Subunit Dissociation for Protein Complexes with Variable Temperature Ion Mobility Mass Spectrometry

Kamila J. Pacholarz and Perdita E. Barran

Leave a Reply

Your email address will not be published.